Research

Resistance to penicillin of Staphylococcus aureus isolates from cows with high somatic cell counts in organic and conventional dairy herds in Denmark

Torben W Bennedsgaard¹ , Stig M Thamsborg² , Frank M Aarestrup³ , Carsten Enevoldsen⁴ , Mette Vaarst¹ and Anna B Christoffersen³

¹  Department of Animal Health, Welfare and Nutrition, Danish Institute for Agricultural Sciences, 8830 Tjele, Denmark

²  Department of Veterinary Pathobiology, The Royal Veterinary and Agricultural University, 1870 Frederiksberg C, Denmark

³  Danish Institute for Food and Veterinary Research, 1790 Copenhagen V, Denmark

⁴  Department of Large Animal Sciences, The Royal Veterinary and Agricultural University, 1870 Frederiksberg C, Denmark

author email corresponding author email

Acta Veterinaria Scandinavica 2006, 48:24doi:10.1186/1751-0147-48-24

Published:	24 November 2006

Abstract

Background

Quarter milk samples from cows with high risk of intramammary infection were examined to determine the prevalence of Staphylococcus aureus (SA) and penicillin resistant SA (SAr) in conventional and organic dairy herds and herds converting to organic farming in a combined longitudinal and cross-sectional study.

Methods

20 conventional herds, 18 organic herds that converted before 1995, and 19 herds converting to organic farming in 1999 or 2000 were included in the study. Herds converting to organic farming were sampled three times one year apart; the other herds were sampled once. Risk of infection was estimated based on somatic cell count, milk production, breed, age and lactation stage.

Results

The high-risk cows represented about 49 % of the cows in the herds. The overall prevalence of SA and SAr among these cows was 29% (95% confidence interval: 24%–34%) and 4% (95% confidence interval: 2%–5%) respectively. The prevalence of penicillin resistance among SA infected cows was 12% (95% confidence interval: 6%–19%) when calculated from the first herd visits. No statistically significant differences were observed in the prevalence of SAr or the proportion of isolates resistant to penicillin between herd groups.

Conclusion

The proportion of isolates resistant to penicillin was low compared to studies in other countries except Norway and Sweden. Based on the low prevalence of penicillin resistance of SA, penicillin should still be the first choice of antimicrobial agent for treatment of bovine intramammary infection in Denmark.