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Chromatin damage in stallion spermatozoa indicated by DFI (DNA fragmentation index, %), its standard deviation (SD_DFI) and the measure of single stranded DNA (mean_DFI). Values are expressed as means ± SD of aliquots of extended ejaculates from eleven stallions (n = 37). |
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| Stallion (no. ejaculates) |
DFI |
SD_DFI |
Mean DFI |
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| A (3) |
5.3 ± 0.5 |
47.7 ± 67.0 |
278.6 ± 9.4 |
| F (4) |
8.7 ± 3.3 |
48.6 ± 6.6 |
280.2 ± 16.2 |
| G (3) |
9.2 ± 1.4 |
48.6 ± 7.0 |
273.9 ± 3.2 |
| H (3) |
17.9 ± 4.5 |
64.0 ± 8.7 |
291.7 ± 13.1 |
| K (4) |
12.5 ± 2.8 |
58.7 ± 4.6 |
267.0 ± 6.5 |
| Q (3) |
5.7 ± 1.1 |
46.3 ± 2.8 |
277.8 ± 4.6 |
| R (3) |
8.4 ± 3.6 |
54.7 ± 6.4 |
283.6 ± 7.3 |
| T (3) |
11.1 ± 6.3 |
56.3 ± 5.3 |
287.5 ± 13.1 |
| W (3) |
13.9 ± 3.0 |
86.5 ± 13.4 |
302.5 ± 14.8 |
| Y (4) |
11.4 ± 2.7 |
56.7 ± 4.1 |
288.8 ± 4.1 |
| Z (4) |
12.6 ± 2.6 |
86.4 ± 8.1 |
297.8 ± 9.7 |
| Overall Mean ± SD |
11.6 ± 13.4 |
56.4 ± 14.4 |
311.0 ± 8.37 |
| Range |
4.8–19.0 |
41.5–98.9 |
267.7–319.5 |
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Notes: DFI gives a measure of the proportion of spermatozoa with damaged chromatin; SD_DFI is the standard deviation of DFI; mean_DFI is a measure of single stranded DNA. Variation between stallions is statistically significant for DFI and SD_DFI (P < 0.001), variation between ejaculates is significant only for mean_DFI (P < 0.05). | |||
Morrell et al. Acta Veterinaria Scandinavica 2008 50:2 doi:10.1186/1751-0147-50-2 |
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