Vancomycin resistant enterococci (VRE) in Swedish sewage sludge

doi:10.1186/1751-0147-51-24

Acta Veterinaria Scandinavica

Volume 51

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Sahlström L
Rehbinder V
Albihn A
Aspan A
Bengtsson B

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Rehbinder V
Albihn A
Aspan A
Bengtsson B
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Research

Vancomycin resistant enterococci (VRE) in Swedish sewage sludge

Leena Sahlström¹^* , Verena Rehbinder²^* , Ann Albihn²^* , Anna Aspan²^* and Björn Bengtsson²^*

¹Finnish Food safety Authority, Evira, Mustialankatu 3, 00790 Helsinki, Finland

²National Veterinary Institute, 75189 Uppsala, Sweden

author email corresponding author email* Contributed equally

Acta Veterinaria Scandinavica 2009, 51:24doi:10.1186/1751-0147-51-24


Published:	29 May 2009

Abstract

Background

Antimicrobial resistance is a serious threat in veterinary medicine and human healthcare. Resistance genes can spread from animals, through the food-chain, and back to humans. Sewage sludge may act as the link back from humans to animals. The main aims of this study were to investigate the occurrence of vancomycin resistant enterococci (VRE) in treated sewage sludge, in a Swedish waste water treatment plant (WWTP), and to compare VRE isolates from sewage sludge with isolates from humans and chickens.

Methods

During a four month long study, sewage sludge was collected weekly and cultured for VRE. The VRE isolates from sewage sludge were analysed and compared to each other and to human and chicken VRE isolates by biochemical typing (PhenePlate), PFGE and antibiograms.

Results

Biochemical typing (PhenePlate-FS) and pulsed field gel electrophoresis (PFGE) revealed prevalence of specific VRE strains in sewage sludge for up to 16 weeks. No connection was found between the VRE strains isolated from sludge, chickens and humans, indicating that human VRE did not originate from Swedish chicken.

Conclusion

This study demonstrated widespread occurrence of VRE in sewage sludge in the studied WWTP. This implies a risk of antimicrobial resistance being spread to new farms and to the society via the environment if the sewage sludge is used on arable land.