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Open Access Highly Accessed Research

Genetic basis of penicillin resistance of S. aureus isolated in bovine mastitis

Arzu Funda Bagcigil12, Suvi Taponen1*, Joanna Koort3, Björn Bengtsson4, Anna-Liisa Myllyniemi5 and Satu Pyörälä1

Author Affiliations

1 Department of Production Animal Medicine, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland

2 Department of Microbiology, Faculty of Veterinary Medicine, University of Istanbul, Istanbul, Turkey

3 Department of Basic Veterinary Sciences, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland

4 National Veterinary Institute, SE-751 89, Uppsala, Sweden

5 Finnish Food Safety Authority Evira, Helsinki, Finland

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Acta Veterinaria Scandinavica 2012, 54:69  doi:10.1186/1751-0147-54-69

Published: 23 November 2012

Abstract

Background

The blaZ gene encoding penicillin resistance can be located either chromosomally or on plasmids. The aim of this study was to investigate the genetic relationships and to determine the location of the blaZ gene in S. aureus isolated in bovine mastitis in Finland and Sweden.

Methods

Seventy-eight β-lactamase positive S. aureus isolates from bovine mastitis (34 from Finland and 44 from Sweden) were included in the study. The localization of blaZ gene was determined by Southern blotting. The blaZ genes of the isolates were sequenced and the sequences were translated to beta-lactamase proteins and further grouped as different protein signatures. The isolates and, as control, 33 Swedish and 36 Finnish beta-lactamase negative isolates were typed with pulsed-field gel electrophoresis (PFGE).

Results

In 26 out of 34 Finnish isolates (76.5%) and in 25 out of 44 Swedish isolates (56.8%) the blaZ gene was localized on a plasmid. Six different protein signatures were found. One signature was found only in four Swedish isolates, but all other signatures were found both in Finnish and Swedish isolates. The PFGE results revealed a diversity of S. aureus clones. The protein signatures were not clearly associated with certain pulsotypes.

Conclusions

The plasmid location of the blaZ gene was not statistically significantly more common in Finland than in Sweden, and hence does not explain the higher proportion of penicillin-resistant isolates of S. aureus causing bovine mastitis in Finland compared to Sweden.

Keywords:
Mastitis; blaZ; Staphylococcus aureus; Beta-lactamases; Penicillin-resistance Correspondence