Reasearch Awards nomination

Email updates

Keep up to date with the latest news and content from Acta Veterinaria Scandinavica and BioMed Central.

Open Access Research

Abattoir-based study on the epidemiology of caprine tuberculosis in Ethiopia using conventional and molecular tools

Benti Deresa123, Franz J Conraths34 and Gobena Ameni1*

Author Affiliations

1 Aklilu Lemma Institute of Pathobiology, Addis Ababa University, PO Box 1176, Addis, Ethiopia

2 Jimma University College of Agriculture and Veterinary Medicine, PO Box 307, Jimma, Ethiopia

3 Friedrich-Loeffler Institute of Epidemiology, Seestraße 5516868, Wusterhausen, Germany

4 Freie Universität Berlin, Koenigsweg 67, Berlin, 14163, Germany

For all author emails, please log on.

Acta Veterinaria Scandinavica 2013, 55:15  doi:10.1186/1751-0147-55-15

Published: 21 February 2013

Abstract

Background

Despite the important role of goats for meat and milk production in Ethiopia, little information is available on the epidemiology of caprine tuberculosis (TB). Caprine TB is important as milk is usually consumed raw particularly by Ethiopian pastoralists. The objectives of the present study were to estimate the prevalence of TB in goats at an abattoir, to evaluate associated risk factors and to characterize the causative mycobacteria.

Methods

A cross-sectional study was conducted on 1990 randomly selected male goats that were slaughtered at Luna Export Abattoir of central Ethiopia. Postmortem examination, mycobacterial culturing and molecular typing techniques like genus typing, deletion typing and spoligotyping were used.

Result

The overall prevalence of caprine TB-like lesions was 3.5%. The lesion prevalence increased significantly with increasing age. Mycobacteria were found by culture and seen as acid fast bacilli in 12% of the goats with TB-like lesions. Characterization of the eight isolates using multiplex polymerase chain reaction (PCR) indicated that five of them belonged to the genus Mycobacterium. Four of the latter were confirmed to be members of the M. tuberculosis complex. Further characterization of the three M. tuberculosis isolates by spoligotyping identified them as type SIT53 and two new spoligotypes.

Conclusion

The isolation of M. tuberculosis from goats in this study indicates a potential risk of transmission of M. tuberculosis between humans and goats.

Keywords:
Goat; Tuberculosis; Epidemiology; Molecular typing; Mycobacterium tuberculosis; Ethiopia