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Open Access Research

Grafts of porcine small intestinal submucosa seeded with cultured homologous smooth muscle cells for bladder repair in dogs

Victor JV Rossetto1*, Lígia SLS da Mota4, Noeme S Rocha2, Hélio A Miot3, Fabrizio Grandi2 and Cláudia VS Brandão1*

Author Affiliations

1 Department of Veterinary Surgery and Anesthesiology, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Rubião Júnior, S/N, Botucatu, ZIP COD 18.618-970, Brazil

2 Department of Clinics, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Rubião Júnior, S/N, Botucatu, ZIP COD 18.618-970, Brazil

3 Department of Dermatology and Radiotherapy, School of Medicine, São Paulo State University (UNESP), Rubião Júnior, S/N, Botucatu, ZIP COD 18.618-970, Brazil

4 Department of Genetic, Institute of Biosciences, São Paulo State University (UNESP), Rubião Júnior, S/N, Botucatu, ZIP COD 18.618-970, Brazil

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Acta Veterinaria Scandinavica 2013, 55:39  doi:10.1186/1751-0147-55-39

Published: 7 May 2013

Abstract

Background

Due to numerous complications associated to gastrointestinal augmented cystoplasty, this study aimed to analyze the anatomic repair of the bladder of 10 female dogs using grafts of porcine small intestinal submucosa (SIS) seeded with cultured homologous smooth muscle cells, and compare them with the acellular SIS grafts.

Results

We assessed the possible side effects and complications of each type of graft by clinical examination, abdominal ultrasound and laboratory findings. Anatomic repair of neoformed bladder was assessed by histological staining for H/E and Masson's Trichrome, analyzed with a Nikon Photomicroscope connected to the system of image analysis Image J.

Conclusions

We propose that SIS associated to homologous smooth cells can improve the quality of tissue repair, and consequently decrease the potential complications inherent to acellular SIS.

Keywords:
Tissue reconstruction; Bioengineered tissue; Cell therapy; SIS